Skip to main content

Illumina Nextera XT DNA Sequencing Library Preparation Kit

Nextera XT DNA Sample Preparation Kit 

enable the researchers to prepare sequence-ready libraries for small genomes, PCR amplicons, and plasmid in 90 minutes. The low amount (1ng) of input DNA makes this method amenable to precious samples available in limited quantities. The prepared library is compatible with all Illumina Sequencing technologies. 

Advantages of Nextera XT Kit include

Fastest and Easiet Sample Prep Workflow
Innovative Sample Normalization
Flexible Multiplexing


Standard Operation Procedure For Preparation of DNA Library

  • Tagmentation Genomic DNA include the fragmentation and tagging of DNA with adapter sequence
  • Amplification of  libraries using limited cycle PCR that adds i7 and i2 adapters and sequences required for sequencing cluster generation
  • Clean up libraries using single sided bead purification to purify amplified beads
  • Library Quality check 
  • Normalization of library quantity 
  • Diluting Library  for the sequencing system
Reference: 


Labels:

Comments

Post a Comment

Popular posts from this blog

Multiple Choice Questions (MCQs) on Diabetes Mellitus: Pathogenesis, Diagnosis and Treatment

                                        MCQs on Diabetes mellitus 1) Diabetes mellitus is a disorder characterized by hyperglycemia.  Which of the following is not the common characteristic features of type 2 diabetes mellitus ? a) Impaired insulin secretion b) Increased Insulin resistance  c) Diabetic ketoacidosis d) Excessive hepatic glucose production 2) Which of the following are the characteristic features of type 1 diabetes mellitus? a) Type 1 diabetes is caused by an absolute deficiency of insulin. b) Type 1 diabetes is associated with the autoimmune destruction of beta cells.  c) Patients with  uncontrolled type 1 diabetes present with diabetic ketoacidosis d) All of the above   3) Which of the following serum measurements are not used for the diagnosis of diabetes mellitus? a) Fasting blood glucose d) Postprandial blood glucose  c) Insulin ...
6 comments
Read More

MCQs on Superficial and Cutaneous Mycoses-Dermatophytes: Medical Mycology

  Multiple Choice Questions on Superficial and Cutaneous Fungal Infections 1. All of the following are examples of common superficial mycoses, EXCEPT ? a) Aspergillosis b) Candidiasis c) Tinea versicolor d) Tinea nigra 2. Which of the following statement regarding Tinea nigra infection is NOT correct? a) Most prevalent in the tropical and subtropical region b) Symptoms include pigmented macules on palm and soles c) Trycophyton spp is the causative fungi d) Common specimen is skin scrapings 3. The fungi Trichosporon commonly causes superficial infection of hair also called white piedra. Which of the following species of Trichosporon causes scalp hair white piedra ? a) T. ovoides b) T. inkin c) T. asahii d) None of the above 4. All of the following are the characteristics of cutaneous mycoses, EXCEPT ? a) The infection of hair, skin, and nails b) The common cause of ringworm in humans only c) Candida spp is associated with the infection d) The keratinize...
3 comments
Read More

MCQs on Human Immunodeficiency Virus (HIV/ AIDS) with answers: Medical Microbiology

Multiple Choice Questions on Human Immunodeficiency Virus (HIV/AIDS) with answers 1) Which virus is associated with acquired immune deficiency syndrome (AIDS) pandemic? A) HIV-2 B) HTLV-1 C) HIV-1 D) HIV-3 2) Which of the following is an important molecule present in the outer membrane of HIV that helps the virus to enter and infect host cells? a) Polysaccharides b) Glycoproteins c) Proteins d) Lipopolysaccharides 3) Select all the incorrect options given below for the common route of transmission of HIV from one person to another? a) Unprotected sexual contact with an infected person b) From infected mother to the fetus c) From the mosquito bite  d) Exposure to contaminated blood and blood products e) Using and sharing the same swimming pool  4) HIV belongs to which of the following genus member of the virus? a) Orthomyxovirus b) Retrovirus c) Parvovirus d) Reovirus 5) All of the following statements regarding HIV infection in human is true, ...
3 comments
Read More