MCQs on equipment used in a microbiology laboratory to analyze samples
1. What is the purpose of an autoclave in a microbiology lab?a) Amplify DNA sequences
b) Measure cell concentration
c) Sterilize equipment and media
d) Visualize microorganisms
2. Which instrument is used to separate components of a microbial sample based on density?
a) Centrifuge
b) Incubator
c) Microscope
d) PCR machine
3. What is the function of a biological safety cabinet?
a) Amplify microbial DNA
b) Provide a sterile environment for microbial growth
c) Separate microbial colonies on agar plates
d) Sterilize growth media, provide a sterile environment while working with microorganisms
4. Which tool is essential for visualizing microorganisms and their structures?
a) Incubator
b) Microscope
c) pH meter
d) Spectrophotometer
5. What is the primary purpose of a laminar flow hood in a microbiology lab?
a) Amplify DNA
b) Create a controlled environment for microbial growth
c) Prevent contamination during work with microorganisms
d) Sterilize equipment
6.Which technique is used to separate DNA, RNA, or proteins based on size and charge?
a) Colony counting
b) Flow cytometry
c) Gel electrophoresis
d) PCR amplification
7. Which equipment is used to measure the amount of light absorbed by microbial cultures?
a) Autoclave
b) Centrifuge
c) Laminar flow hood
d) Spectrophotometer
8. What is the primary purpose of a PCR machine in a microbiology lab?
a) Amplifying DNA sequences
b) Microbial identification
c) Measuring pH levels
d) Sterilization
9. Which of the following statement is correct regarding the refrigerators used for the specimen storage?
a) Microbial specimens are only stored at 4°C
b) Microbial specimens are stored at 4°C, or kept frozen -20°C and -70°C
c) Microbial specimens are kept at 22°C
d) Refrigerators are not used for the specimen storage
10. What is the purpose of a flow cytometer in microbiology?
a) Sterilize equipment
b) Amplify DNA
c) Count microbial colonies
d) Analyze individual cells based on characteristics
a) Autoclave
b) Microbial incubator
c) PCR machine
d) Spectrophotometer
13. Which tool is used to identify microorganisms based on their metabolic characteristics?
a) PCR machine
b) Laminar flow hood
c) Microbial identification system
d) Gel electrophoresis system
14. What is the primary function a Bunsen burner in a microbiology laboratory?
a) Count microbial colonies
b) Provide heat and sterilize the equipment
c) Create a controlled environment for microbial growth
d) None of the above
15. Which of the following equipment is used for incubation of samples at specific temperatures?
a) Centrifuge
b) Fume hoods
c) Flow cytometer
d) Water baths
a) Autoclave
b) Flow cytometer
c) Incubator
d) Spectrophotometer
a) Amplifying DNA
b) Counting microbial colonies
c) Separating DNA fragments based on size
d) Sterilization
18. Which instrument is used to measure the growth of microbial cultures by analyzing light scattering?
a) Centrifuge
b) pH meter
c) PCR machine
d) Spectrophotometer
19. What is the function of a cryogenic storage system in a microbiology lab?
a) Sterilize equipment
b) Amplify DNA
c) Grow microbial cultures
d) Store microorganisms at ultra-low temperatures
Answers with explanation:
1. c) Sterilize equipment and media. Autoclave uses a high-pressure steam to achieve sterilization, effectively killing microorganisms and ensuring that cultures, media, and equipment are free from contamination.
2. a) Centrifuge. Centrifuges are used to separate microbial cells from the growth media or suspension they are in.
3. d) Sterilize growth media, provide a sterile environment while working with microorganisms
4. b) Microscope
5. c) Prevent contamination during work with microorganisms
6. c) Gel electrophoresis. A common technique usually used in biochemistry and molecular biology lab that allows separation and analysis of DNA, RNA, and proteins, can move through a semisolid gel matrix when an electric field is applied.
7. d) Spectrophotometer. It is used to quantify the amount of light absorbed, transmitted, or reflected by a sample.
8. a) Amplifying DNA sequences
9. b) Microbial specimens are stored at 4°C, or kept frozen -20°C and -70°C
10. d) Analyze individual cells based on characteristics
11. c) Light microscope, consists of visible light to illuminate specimens that allows laboratory person to view and observe internal structures of microorganisms and other biological samples.
12. b) Microbial incubator. They provide a controlled environment with specific temperature and humidity settings to promote the growth of microorganisms under optimal conditions.
13. c) Microbial identification system
14. b) Provide heat and sterilize the equipment. It is used to sterilize equipment like inoculating loops or needles by passing them through the flame, this prevents cross-contamination during experiments.
17. c) Separating DNA fragments based on size. Gel electrophoresis allows researchers to analyze and characterize these molecules.
18. d) Spectrophotometer. The equipment measures the amount of light absorbed or scattered by microbial cultures, this measurement is used to estimate the concentration of cells in a culture and monitor their growth.
19. d) Store microorganisms at ultra-low temperatures. Cryogenic storage systems are used for the for storage of microorganisms and cell lines at extremely low temperatures, often using liquid nitrogen, to preserve their viability for long periods.
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