MCQs on Biological Safety Level (BSL) and Use of Biological Safety Cabinets (BSCs) in a laboratory: Medical microbiology
MCQs on Biological Safety Level
1. What does BSL stand for in laboratory safety?
a. Biosecurity Level
4. At which BSL would you expect to find work with microorganisms that pose minimal risk to laboratory personnel and the environment?
a. BSL-1
b. BSL-2
c. BSL-3
d. BSL-4
5. Which BSL is typically used for research involving agents that can cause serious or potentially lethal diseases, and where there is a higher risk of exposure?
a. BSL-1
b. BSL-2
c. BSL-3
d. BSL-4
6. In a BSL-2 laboratory, what type of protective clothing is typically required for laboratory personnel?
a. Lab coat and gloves
b. Lab coat, gloves, and safety goggles
c. Lab coat, gloves, safety goggles, and a face mask
d. Lab coat, gloves, safety goggles, and a face shield
7. What is the primary purpose of BSL-4 containment?
a. To protect the environment from contamination
b. To ensure the safety of laboratory personnel
c. To prevent the release of microorganisms into the community
d. To conduct research on the most dangerous pathogens
d. BSL-4
d. BSL-4
2) a. CDC (Centers for Disease Control and Prevention)
3) b. To maintain a sterile environment. Biological Safety Cabinets (BSCs) are designed to prevent the release of harmful biological agents, such as bacteria, viruses, and other microorganisms, into the laboratory environment.
5) c. BSL-3. BSL-3 laboratories handle microorganisms that can cause serious or potentially lethal diseases in humans.
6) b. Lab coat, gloves, and safety goggles
7) d. To conduct research on the most dangerous pathogens.
8) d. HEPA filter. High-Efficiency Particulate Air (HEPA) filters, which capture and remove airborne particles down to 0.3 micrometers in size. These filters help to maintain a sterile environment within the cabinet.
10) b. They involve high-risk pathogens with no available vaccines or treatments.
11) c. The pathogens or agents being handled
12) d. BSL-4
BSL (Biosafety Level): BSL is a system of classification that categorizes laboratories and procedures based on the level of containment and protection required when working with various biological agents. BSL levels range from 1 (lowest risk) to 4 (highest risk).
BSC (Biological Safety Cabinet): BSCs are specialized laboratory equipment designed to provide containment and protection for individuals working with biological materials, primarily to prevent the release of hazardous agents into the environment and protect the operator.
1. What does BSL stand for in laboratory safety?
a. Biosecurity Level
b. Biosafety Laboratory Standard
c. Biological Safety Level
d. Biotechnology Safety Level
2. Which organization developed the Biosafety Levels (BSL) to classify and regulate the handling of hazardous biological materials in laboratories?
a. CDC (Centers for Disease Control and Prevention)
b. EPA (Environmental Protection Agency)
c. OSHA (Occupational Safety and Health Administration)
d. WHO (World Health Organization)
c. Biological Safety Level
d. Biotechnology Safety Level
2. Which organization developed the Biosafety Levels (BSL) to classify and regulate the handling of hazardous biological materials in laboratories?
a. CDC (Centers for Disease Control and Prevention)
b. EPA (Environmental Protection Agency)
c. OSHA (Occupational Safety and Health Administration)
d. WHO (World Health Organization)
3. What is the primary purpose of a biological safety cabinet (BSC)?
a. To provide lighting for the laboratory
b. To maintain a sterile environment
c. To regulate temperature and humidity
d. To store chemicals safely
4. At which BSL would you expect to find work with microorganisms that pose minimal risk to laboratory personnel and the environment?
a. BSL-1
b. BSL-2
c. BSL-3
d. BSL-4
5. Which BSL is typically used for research involving agents that can cause serious or potentially lethal diseases, and where there is a higher risk of exposure?
a. BSL-1
b. BSL-2
c. BSL-3
d. BSL-4
6. In a BSL-2 laboratory, what type of protective clothing is typically required for laboratory personnel?
a. Lab coat and gloves
b. Lab coat, gloves, and safety goggles
c. Lab coat, gloves, safety goggles, and a face mask
d. Lab coat, gloves, safety goggles, and a face shield
7. What is the primary purpose of BSL-4 containment?
a. To protect the environment from contamination
b. To ensure the safety of laboratory personnel
c. To prevent the release of microorganisms into the community
d. To conduct research on the most dangerous pathogens
8. Which type of filter is commonly used in BSCs to capture and remove airborne particles?
9. Which BSL level is used for work with microorganisms that are not known to consistently cause disease in healthy adults?
a. BSL-1
b. BSL-2
c. BSL-3
d. BSL-4
10. Which of the following is true regarding BSL-3 laboratories?
a. They are designed for routine clinical testing.
b. They involve high-risk pathogens with no available vaccines or treatments.
c. They do not require specific safety precautions.
d. They are the most common type of laboratory setting.
11. What is the primary factor that determines the BSL classification of a laboratory?
a. The laboratory's location
b. The laboratory's funding source
c. The pathogens or agents being handled
d. The size of the laboratory
12. Which of the following BSL is characterized by the highest level of containment and is used for work with dangerous pathogens that have no known treatments or vaccines?
a. BSL-1
b. BSL-2
c. BSL-3
d. BSL-4
a. Carbon filter
b. Electrostatic filter
c. Glass fiber filter
d. HEPA filter
9. Which BSL level is used for work with microorganisms that are not known to consistently cause disease in healthy adults?
a. BSL-1
b. BSL-2
c. BSL-3
d. BSL-4
10. Which of the following is true regarding BSL-3 laboratories?
a. They are designed for routine clinical testing.
b. They involve high-risk pathogens with no available vaccines or treatments.
c. They do not require specific safety precautions.
d. They are the most common type of laboratory setting.
11. What is the primary factor that determines the BSL classification of a laboratory?
a. The laboratory's location
b. The laboratory's funding source
c. The pathogens or agents being handled
d. The size of the laboratory
12. Which of the following BSL is characterized by the highest level of containment and is used for work with dangerous pathogens that have no known treatments or vaccines?
a. BSL-1
b. BSL-2
c. BSL-3
d. BSL-4
13. Which of the following BSL is recommended for the handling of samples suspected of Mycobacterium tuberculosis?
a. BSL-1
b. BSL-2
c. BSL-3d. BSL-4
14. Which class of biological safety cabinet provides the highest level of containment and is used for working with highly hazardous materials?
a. Class I BSC
b. Class II BSC
c. Class III BSC
d. Class IV BSC
15. Laboratory samples suspected of containing organism and that would generate aerosols should be handled in which of the following BSL ?
a. BSL-1
b. BSL-2
c. BSL-3d. BSL-4
16. Specimens of Ebola and Marburg virus for cell culture identification should be handled in which of the following BSL?
a. BSL-1
d. BSL-4
Answers:
1) c. Biological Safety Level. BSL stands for "Biological Safety Level." It is a system of containment and safety measures used in laboratory settings to control the risk associated with handling various biological agents, such as microorganisms, viruses, and toxins. BSLs are designed to protect laboratory personnel, the environment, and the community from potential hazards posed by these biological agents.
b. BSL-2
c. BSL-3d. BSL-4
17. What is the purpose of the UV germicidal lamp in some BSCs?
a. To provide additional lighting
b. To sterilize the operator's hands
c. To sterilize the work surface and contents
d. To maintain a controlled temperature
1) c. Biological Safety Level. BSL stands for "Biological Safety Level." It is a system of containment and safety measures used in laboratory settings to control the risk associated with handling various biological agents, such as microorganisms, viruses, and toxins. BSLs are designed to protect laboratory personnel, the environment, and the community from potential hazards posed by these biological agents.
2) a. CDC (Centers for Disease Control and Prevention)
3) b. To maintain a sterile environment. Biological Safety Cabinets (BSCs) are designed to prevent the release of harmful biological agents, such as bacteria, viruses, and other microorganisms, into the laboratory environment.
4) a. BSL-1. BSL-1, or Biological Safety Level 1, is the lowest containment level in the biosafety levels hierarchy.
5) c. BSL-3. BSL-3 laboratories handle microorganisms that can cause serious or potentially lethal diseases in humans.
6) b. Lab coat, gloves, and safety goggles
7) d. To conduct research on the most dangerous pathogens.
8) d. HEPA filter. High-Efficiency Particulate Air (HEPA) filters, which capture and remove airborne particles down to 0.3 micrometers in size. These filters help to maintain a sterile environment within the cabinet.
9) a. BSL-1. BSL-1, or Biological Safety Level 1, is the lowest containment level in the biosafety levels hierarchy.
10) b. They involve high-risk pathogens with no available vaccines or treatments.
11) c. The pathogens or agents being handled
12) d. BSL-4
13) c. BSL-3. M. tuberculosis is considered a high-risk pathogen that can lead to severe and potentially life-threatening diseases in humans. BSL-3 prevents the accidental release of M. tuberculosis or other similar high-risk pathogens into the environment.
14) c. Class III BSC. These laboratory equipment are typically used in laboratories and facilities that handle the most dangerous pathogens and biological materials, including those that can cause severe diseases or pose a significant risk to public health.
15) c. BSL-3
16) d. BSL-4. Microorganisms that poses highest risk, can cause severe diseases with no known treatments or vaccines, and transmission can occur easily are handled in BSL-4.
17) c. To sterilize the work surface and contents. Some BSCs include built-in ultraviolet (UV) germicidal lamps, which can be used to sterilize the work surface and contents when not in use.
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